For our research activities we make use of all modern methods of medical biotechnology. Here you can find an overview of the

Methods used:

• Molecular-biological methods
  • Preparation and purification of oligonucleotides
  • Polymerase chain reaction
  • Cloning into different vector systems
  • Transformation of different hosts for expression

• Antibody technologies
  • Immunization strategies
  • Hybridoma technology
  • EBV-Transformation of human B-lymphocytes
  • Phage Display
  • In vitro- / ex vivo-selection strategies

• Protein-based methods
  • Protein engineering
  • Expression in different hosts
  • Protein purification
  • SDS-gel electrophoresis and Western blotting

• In vitro-assays
  • ELISA
  • Resonance spectrometry
  • Flow cytometry
  • Fluorescence-/Confocal microscopy
  • Cytotoxicity-/Apoptosis assays

Diagnostics

• In vitro
  • ELISA: Sandwich & direct (Serum analysis)
  • Immunohistochemistry
  • Immunocytology
  • Single cell analyses
• In vivo
  • Optical imaging
  • Antibody-based disease-specific contrast agents

Therapeutics

• Fusion proteins based on bacterial toxins (immunotoxins)
  • Antibody based (cancer and chronic inflammation)
  • Antigen based (autoimmune diseases and allergies)

• Completely human cytolytic fusion proteins (bacterial toxins replaced by human enzymes)
  • Proteases
  • RNases
  • Kinases
  • Mikrotubule-associated proteins